Journal of Clinical Immunology and Allergy

The Gtpase Rab1 is Required for NLRP3 Inflamma some Activation and Inflammatory Lung Injury

Rationale: Uncontrolled inflammatory response in sepsis predominantly contributes to development of multiorgan failure and lethality. However, the cellular and molecular mechanisms for excessive production and release of proinflammatory cytokines are not clearly defined. The purpose of this study is to determine the role of the GTPase Ras-related protein in brain (Rab)1a in regulating the nucleotide binding domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation and lung inflammatory injury during sepsis. Methodology: Human alveolar macrophages were isolated from septic patients. Cecal ligation and puncture was performed to assess lung inflammation. Genetic manipulation of Rab1 expression in murine bone marrow-derived macrophages was conducted by electroporation

Author(s):

Lijun Wang

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